Ambra de Simone - Biochemistry: Ascorbate extraction.

Ambra de Simone

Biochemistry: Ascorbate extraction.

Postdoctoral Researcher

University of Leeds

Description

I specialise in working and handling early germinated Arabidopsis seeds. I perform total and reduced ascorbate plate reader assay. In vivo glutathione redox potential measurements using redox-sensitive probes such as roGFP2 (redox sensitive GFP). I can also provide molecular biology services such as high-quality RNA extraction from Arabidopsis seeds.

I'm looking forward to working with you!

What do you need to provide us with?

I perform ascorbate assay from frozen leaves tissues (minimum 80 mg).
For the extraction of RNA from seeds I would need 10-20mg of Arabidopsis seeds

What will I deliver?

Ascorbate assays: Excel file with micromol per g FW calculated.
RNA extraction: 50 ul/10 mg of seeds of high-quality total RNA from dry seeds delivered on dry ice through the courier of your choice.

Publications


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Article: Glutathione - Linking cell proliferation to oxidative stress

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Article:

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